|"PROT-SSA" represents the reaction observed on the sulfosalicylic acid (SSA) precipitation test. The SSA reagent is added to a small volume of urine. Acidification causes precipitation of protein in the sample (seen as increasing turbidity), which is subjectively graded as trace, 1+, 2+, 3+ or 4+.|
Unlike the "PROT-STIX" test, the SSA reaction will detect albumin and globulins (although it is more sensitive to albumin). In addition, the SSA detects Bence-Jones proteins, although it often underestimates them.|
In alkaline urine, the SSA reaction is a more accurate measure of urine protein content than the dipstick.
The most accurate measurement of urine protein output is measurement of urine protein excretion over 24-hours. A good alternative to this test is the urine protein to creatinine ratio.